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Although the incidence of basal or spontaneous membrane lipid peroxidation was low during liquid storage and after freeze-thawing, viable boar sperm were susceptible to FeAc-induced lipid peroxidation. Basal BODIPY oxidation (no FeAc) was low in liquid stored and thawed viable sperm (1.3 and 3.4%, respectively). Experiments were conducted to determine the effect of liquid storage at 17☌ for 1 and 5 d and the effect of freeze-thawing on basal and FeAc-induced BODIPY oxidation. The presence of the metal chelators EDTA or deferoxamine mesylate at 3 and 9 μ M inhibited FeAc-induced BODIPY oxidation and maintained motility. The oxidization of hydroethidine to ethidium was specific for menadione and H 2O 2 FeAc had no effect. Of the reactive oxygen species generators tested, BODIPY oxidation was specific for FeAc, because menadione and H 2O 2 had little or no effect. Motility was depressed ( P < 0.05) after exposure to FeAc, but viability was not affected. Compared with no FeSO 4 and Na ascorbate, the combination of FeSO 4 and Na ascorbate (FeAc) increased ( P 70% and increased ( P < 0.05) BOD-IPY fluorescence intensity/cell by 5- to 10-fold after a 30-min incubation. Experiments were conducted with Percoll-washed sperm to determine the specificity of BODIPY oxidation in the presence of different reactive oxygen species generators and metal chelators. Lipid peroxidation was monitored by the shift from red to green fluorescence emission of the lipophilic probe 4, 4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid, C 11BODIPY 581/591 (BODIPY), as measured by fluorescence-activated flow cytometry in live sperm (negative for propidium iodide).
MINITUBE BTS CODE
Plastic stem tube for cow sperm, red, packed 2, 000 pieces / bag, code 13407/0044.â minitube brand.Part of the reduction in boar sperm motility and fertility associated with hypothermic liquid storage and cryopreservation may be due to membrane lipid peroxidation. Plastic stem tube for cow sperm, yellow, packed 2, 000 pieces / bag, code 13407/0094.â minitube brand.â 100% new Refined dye for sperm screening, code 15405/0026, 1 set consisting of 3 bottles of x250ml.â minitube brand.â 100% newĪndromed beef phase concentrate medium, 200ml capacity.â ref code: 13503/0200.â minitube brand.â 100% new Plastic billet protection tool used in the laboratory: 16912/0133 - goblets diam.10mm, nsx: minitube, 100% new. Laboratory plastic billet protection tools: 16980/0601 - multipurpose cassette, short version, 140mm, nsx: minitube, 100% new. Makeup base set with loreal paris gwp infallible minitube gwp (makeup foundation + plastic bag) Minitube brand plastic bags (used in veterinary medicine) 100% brand new.â 13210/0106, 5l